What is the purpose of carbol fuchsin?

Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells wall lipids than in the acid alcohol.

Why heated carbol fuchsin is used in acid-fast staining?

The primary stain used in acid-fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat in the form of heat (steam).

Why is the slide needed to be heated after the application of carbol fuchsin?

When the smear is stained with carbol fuchsin, it solubilizes the lipoidal material present in the Mycobacterial cell wall but by the application of heat, carbol fuchsin further penetrates through lipoidal wall and enters into cytoplasm.

How long do you flood the slide with carbol fuchsin in an acid-fast stain?

Acid-Fast Staining Instructions

1. Air dry and heat fix a thin film of microorganisms.
2. Flood the slide with Carbolfuchsin.
3. Flood slide with Acid Alcohol for 30 seconds.
4. Counterstain by flooding the slide with Methylene Blue for 30 seconds.
5. Dry the slide by putting it between the pages of a book of Bibulous paper.

Why is steaming carbol fuchsin a necessary step in Ziehl-Neelsen staining?

The phenolic compound carbol fuchsin is used as the primary stain because it is lipid soluble and penetrates the waxy cell wall. Staining by carbol fuchsin is further enhanced by steam heating the preparation to melt the wax and allow the stain to move into the cell.

Why is phenol added to the carbol fuchsin stain for acid-fast staining quizlet?

(Fuchsin is more soluble in phenol (carbolic acid) than in water, and phenol solubilizes more easily in lipids than in acid alcohol. Therefore, carbolfuchsin has a higher affinity for lipids than for acid-alcohol and will remain with the cell wall when washed with acid-alcohol. How do you perform an acid-fast stain?

Why is phenol added to the carbol Fuchsin stain for acid fast staining quizlet?

Is carbol Fuchsin acidic or basic?

Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores.

Why is it important to steam the slides in the Ziehl-neelsen method?

the Ziehl-Neelsen method uses steam, Kinyoun method does not. what makes a bacteria acid-fast? why is steaming the slide important? heating the slide “melts” or increases the fluidity of acid-fast cells and permits the penetration of the primary stain.

Why must heat be used with the application of the primary stain during acid fast staining?

During the acid fast stain, heat is used as a mordant to allow the primary stain to penetrate the waxy mycolic acid layer. The heat will prevent the cells from being destained using acid-alcohol. Because these cells hold fast to the primary stain with acid alcohol treatment, they are termed acid fast positive.

How do you make strong carbol Fuchsin?

Instructions for preparation of 1\% Carbol fuchsin: Using a digital balance weigh out 1 g of Basic fuchsin in a sterile 100 ml flask. 2. Add 100 ml of absolute alcohol and dissolve the dye by placing it in a water bath at 60° C. Avoid direct heating (Solution 1).

Why was it important to steam the slides in the Ziehl-neelsen method?