Table of Contents
- 1 Where is the smallest segment of DNA in agarose gel electrophoresis?
- 2 Which lane contains the smallest fragment of DNA?
- 3 How does the process of gel electrophoresis separate DNA fragments?
- 4 What size fragments would you expect to move toward the opposite end of the gel most rapidly explain?
- 5 Why do smaller DNA fragments migrate through the agarose gel more quickly than larger DNA fragments?
- 6 Why measuring sizes of DNA fragments can be useful?
Where is the smallest segment of DNA in agarose gel electrophoresis?
The smallest segment of DNA are found near the positive electrode farthest away from the wells.
Which DNA fragment is the shortest?
E
The shortest strand of DNA is E and the longest strand of DNA is B. The agarose gel used in gel electrophoresis has tiny pores throughout.
Which lane contains the smallest fragment of DNA?
Lane 6 has the smallest fragment because it has a piece the furthest away from the top of the KB ladder. The further away you are from the top the smaller of a fragment you can have. Lane 2 and 4 have the largest because they have pieces closer to the top of the KB ladder.
How does the DNA rate of travel differ for small DNA fragments and large DNA fragments?
How does the DNA rate of travel differ for small DNA fragments and large DNA fragments? Small fragments travel farther than large fragments. A high voltage rate will cause the DNA fragments to move slowly across the gel. A DNA fragment with 100 base pairs is smaller than a DNA fragment with 150 base pairs.
How does the process of gel electrophoresis separate DNA fragments?
How does the process of gel electrophoresis separate DNA fragments? It uses an electric current to separate different sized molecules of DNA in a porous sponge-like matrix.
How does DNA move in gel electrophoresis?
Gel electrophoresis and DNA DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
What size fragments would you expect to move toward the opposite end of the gel most rapidly explain?
What size fragments would you expect to move toward the opposite end of the gel most quickly? The small fragments because they are entangled less in the agarose gel.
Where are the smallest DNA fragments located on the gel?
The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode).
Why do smaller DNA fragments migrate through the agarose gel more quickly than larger DNA fragments?
An electric current is applied across the gel so that one end of the gel has a positive charge and the other end has a negative charge. Smaller molecules migrate through the gel more quickly and therefore travel further than larger fragments that migrate more slowly and therefore will travel a shorter distance.
How do you determine the size of DNA fragments?
Using electrophoresis, we can see how many different DNA fragments are present in a sample and how large they are relative to one another. We can also determine the absolute size of a piece of DNA by examining it next to a standard “yardstick” made up of DNA fragments of known sizes.
Why measuring sizes of DNA fragments can be useful?
Knowing the bp length of a DNA fragment can be essential when working with repetitive DNA regions like microsatellites, when constructing recombinant DNA plasmids, or when collecting information for large databases. In many cases, length can also provide added evolutionary information.