In which condition is ara operon functional?

In which of this condition is Ara operon functional? Explanation: When repressor binds to I1 and I2 then RNA polymerase faces no hindrance in binding to the promoter and the genes are turned on. On the other hand when induces the repressor bind to O2 and I1 which leads to the looping out and turning off.

What feature of the arabinose operon is particularly unique?

The mechanism of riboswitch regulation involves short ribonucleotide sequence elements that are capable of binding with small molecule ligands. Which feature of the arabinose operon is particularly unusual? It is an inducible operon in which the same regulatory protein can exert both positive and negative control.

How is arabinose operon different from other operons?

At first glance, this operon seems to be similar to the lac operon. When arabinose is absent, AraC is produced and gets attached to araC. In this way araC acts as its own regulator. While lac operon is usually negatively regulated, ara operon is both positively and negatively regulated, depending on circumstances.

How does arabinose interact with the AraC protein help to turn on the RFP gene?

When arabinose is present, it binds to araC protein. RNA polymerase then binds to this complex & can then transcribe the rfp gene.

What does arabinose do to bacteria?

Thus when arabinose is present to turn on the arabinose operon, GFP is produced and the bacteria can fluoresce. Without arabinose,the GFP gene is not expressed and there is no fluorescence. So by varying nutrient composition, a researcher can control when (and if) florescence occurs in the transformed bacteria.

What is the purpose of arabinose?

L-Arabinose is a constituent of many biopolymers that make up plant cell walls. This monosaccharide is often used in cell culture media, serving as a bacterial carbon source, and can be used to distinguish between bacteria based on their fermentation abilities.

What is the role of arabinose in bacterial transformation?

In other words, when arabinose is in the environment, these genes are turned on. When arabinose runs out, these genes are turned back off. When bacteria transformed with pGLO are grown in the presence of arabinose, the GFP gene is turned on and the bacteria fluoresce a brilliant green color.

What is the regulatory gene for the arabinose operon?

Regulatory gene The ara-C is the only regulatory gene in the arabinose operon. The ara-C gene encodes the Ara C protein that acts as a repressor. The Ara C protein regulates the arabinose operon both positively and negatively.

How does the ara-operon system provide energy to the cell?

Ara-operon system provides energy to the cell by the breakdown of arabinose into xylulose 5-phosphate. Arabinose is a 5-C sugar or aldopentose that provides energy in a carbon source to the bacterial cells. Xylulose 5-phosphate is a ketose sugar and serves as an intermediary product of the pentose phosphate pathway.

How does arabinose pGLO work?

Arabinose creates a differential medium, which means that both pGLO and non-pGLO cells can grow, but they look different (only the pGLO cells become fluorescent). Additionally, how does the arabinose pGLO operon work? The AraC protein binds to the DNA at the binding site for RNA polymerase (the beginning of the arabinose operon).

Why do bacteria fluoresce when arabinose is present?

The plasmid naturally contains an operon for arabinose digestion. if arabinose is present in nutrient medium, the operon’s structural genes for arabinose digestion will be turned on. Thus when arabinose is present to turn on the arabinose operon, GFP is produced and the bacteria can fluoresce. What does pGLO stand for? Why does pGLO glow?