What is toluidine blue O used for?

Toluidine blue is often used to identify mast cells, by virtue of the heparin in their cytoplasmic granules. It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage.

What is P-toluidine used for?

Used in dyes, and in organic chemical manufacturing. P-toluidine is an aminotoluene in which the amino substituent is para to the methyl group.

Where does Toluidine come from?

o-Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation.

What is toluidine blue made of?

The dyes exhibiting metachromatic properties are mainly of thiazine group, thionine, TB, azure A, azure B, methyl violet, safranin, and acridine orange. [4] TB is one such dye, which has been used in tissue sections for identification of various cells.

What is metachromatic dye?

A dye—e.g., methylene blue, toluidine blue, safranine—capable of staining tissues with colours that differ from the dye itself.

Does toluidine blue expire?

Shelf-life Toluidine blue O (C.I. 52040) – for microscopy Certistain® can be used until the stated expiry date. After first opening of the bottle, the contents can be used up to the stated expiry date when stored at +5 °C to +30 °C.

What is difference between toluene and toluidine?

is that toluene is (organic compound) a colourless, inflammable liquid hydrocarbon, methylbenzene, ch3c6h5, used as a solvent, in high-octane fuels and in the production of many chemical compounds while toluidine is (organic compound) any of the three isomeric aromatic amines derived from toluene; they are used in the …

Is para toluidine saturated?

Para toluidine is the isomer of toluidine and is an aryl amine in which a methyl group is present at the para position of an Aniline. It is aromatic and unsaturated because of the …

What color is O-Toluidine?

colorless
O-toluidine appears as a clear colorless or light yellow liquid. May become reddish brown on exposure to air and light. Flash point 185°F. Has about the same density as water and is very slightly soluble in water.

Is toluidine toxic?

o-Toluidine is primarily used in the manufacture of dyes. o-Toluidine is highly toxic to humans when absorbed through the skin, inhaled as vapor, or swallowed. Acute (short-term) exposure of humans to o- toluidine affects the blood (i.e., methemoglobinemia), with clinical signs of central nervous system depression.

What is mast cell?

Listen to pronunciation. (mast sel) A type of white blood cell that is found in connective tissues all through the body, especially under the skin, near blood vessels and lymph vessels, in nerves, and in the lungs and intestines.

Is toluidine blue harmful?

It is an eye irritant. Its toxicity has not been quantified but has proven to be highly toxic to laboratory animals when injected intravenously. Other dyes in this group are known to be highly toxic, e.g. methylene blue. If irritation persists seek immediate medical attention.

What are the medical uses of o-toluidine?

Hair stylists because o-toluidine is used in some hair dyes. Laboratory workers who use o-toluidine to stain tissues or as part of a reagent to analyze glucose. PrilocaineExternal, a cream that is applied to the skin to numb it for minor dental and surgical procedures and blood draws.

What is the color of o-toluidine?

O-toluidine appears as a clear colorless or light yellow liquid. May become reddish brown on exposure to air and light. Flash point 185°F. Has about the same density as water and is very slightly soluble in water. Vapors are heavier than air.

What is p-toluidine made of?

P-toluidine is a colorless solid. Melting point 44°C (111°F). Specific gravity 1.046. Vapor heavier than air. Produces toxic oxides of nitrogen during combustion. May be absorbed through the skin. Used in dyes, and in organic chemical manufacturing. P-toluidine is an aminotoluene in which the amino substituent is para to the methyl group.

What is ortho-toluidine and is it dangerous?

ortho -Toluidine (CH 3 C 6 H 4 NH 2) is a colorless to pale-yellow liquid. It is a toxic substance harmful to the eyes, skin, and when swallowed or inhaled. ortho -Toluidine can cause cancer. Workers may be harmed from exposure to ortho -toluidine. The level of exposure depends upon the dose, duration,…