How do you test for the presence of aspirin?

Acetylsalicylic acid (ASA) is still one of the most commonly used therapeutic agents. Salicylic acid, the major metabolite of ASA, can be detected easily in urine using simple chemical spot tests such as ferric chloride or Trinder’s reagent.

How aspirin can be Analysed Colorimetrically?

The amount of 2-hydroxybenzoic acid in a solution made by hydrolysing aspirin can be determined by adding iron(III) ions and measuring the intensity of the violet-blue solution. From this the amount of aspirin can be calculated.

What wavelength should be used for the detection of aspirin via spectrophotometry?

530 nm
Sample methods for Spectrophotometric Analysis of Aspirin The absorbance and transmittance of the five solutions were measured at 530 nm. The mass of ASA in commercial pain reliever tablets was determined using spectrophotomeric analysis.

How is aspirin prepared in laboratory?

Aspirin is prepared by chemical synthesis from salicylic acid, through acetylation with acetic anhydride. The molecular weight of aspirin is 180.16g/mol. It is odourless, colourless to white crystals or crystalline powder.

What is the purity of aspirin?

The melting point range for the acetylsalicylic acid obtained is 144°C-172°C, it is because it contains impurities. The theoretical yield of aspirin is 2.628g, but the actual yield of aspirin obtained is 0.8953g. therefore, the percentage yield of aspirin is 34.07\%.

What is ASA blood test?

This is a blood test to check for salicylate intoxication, which is usually caused by an overdose of aspirin. This test is also used to check for the correct aspirin dose in people who are given high doses of aspirin to treat inflammation from arthritis. Aspirin contains acetylsalicylic acid (ASA).

What is the role of the FeCl3 KCl HCl solution in the experiment?

FeCl3-KCl-HCl solution is used instead of water because the aspirin ferric ion complex contained FeCl3-KCl-HCl and ASA, but we only want to test the absorbance of ASA, not anything else. By using FeCl3-KCl-Hcl as the reference , we are taking that solutions absorptivity into account.

What is the spectrophotometric analysis of aspirin?

SPECTROPHOTOMETRIC ANALYSIS OF ASPIRIN. Introduction: A colored complex is formed between aspirin and the iron (III) ion. The intensity of the color is directly related to the concentration of aspirin present; therefore, spectrophotometric analysis can be used.

How is aspirin hydrolyzed?

Aspirin Is Hydrolyzed in Plasma, Hydrolysis Is Highly Variable, and Extracellular Hydrolysis Affects Platelet Inhibition. Aspirin was hydrolyzed in cell-free plasma but with considerable variation. The rate of hydrolysis ranged by 7-fold from 3.67 to 24.70 nmol/ml/min among 11 healthy donors (Fig. 1A).

What are the properties of aspirin?

Aspirin is an orally administered non-steroidal antiinflammatory agent. Acetylsalicylic acid binds to and acetylates serine residues in cyclooxygenases, resulting in decreased synthesis of prostaglandin, platelet aggregation, and inflammation. This agent exhibits analgesic, antipyretic, and anticoagulant properties.

Would pure aspirin give a positive FeCl3 test?

Iron (III) ion reacts with phenols to form a purple complex. Salicylic acid contains a phenol group, but acetylsalicylic acid does not. Therefore, if you add FeCl3 to an aspirin sample and you see a purple color, it means that there is still some salicylic acid present and the sample is impure.

How do you test for aspirin with salicylic acid?

1. Label three test tubes; place a few crystals of salicylic acid into test tube #1, a small sample of your aspirin into test tube #2, and a small sample of crushed commercial aspirin into #3. Add 5 mL of deionized water to each test tube and swirl to dissolve the crystals.

How do you test for aspirin with ferric chloride?

PROCEDURE Label three test tubes; place a few crystals of salicylic acid into test tube #1, a small sample of your aspirin into test tube #2, and a small sample of crushed commercial aspirin into #3. Add 5 mL of deionized water to each test tube and swirl to dissolve the crystals. Add 10 drops of 1\% ferric chloride to each test tube.

Should aspirin and blood thinners be taken together?

However, the perception that aspirin by itself is sufficient or that the risk of aspirin plus a blood thinner is not worth the benefit may also be driving forces. Both researchers said more studies evaluating cardiovascular outcomes in AF patients prescribed aspirin-only versus oral blood thinners (or in combination) are needed.

How do you use a capillary tube to dissolve aspirin?

Obtain a capillary tube from your instructor, and gently press the open end into the pile of aspirin crystals on the paper so that a few crystals of aspirin enter the capillary tube. 2. Tap the closed end of the capillary onto the bench top, so that the aspirin crystals work their way to the bottom.