Table of Contents
- 1 What is the basic equipment required for cell culture?
- 2 How much does a tissue culture incubator cost?
- 3 What is the primary equipment required for animal tissue culture laboratory?
- 4 How do you start a cell culture?
- 5 How do you decontaminate a tissue culture hood?
- 6 Why is there 5 CO2 in cell culture incubator?
What is the basic equipment required for cell culture?
What are the Basic equipments required for animal cell culture?
| Essential equipments | Beneficial equipments | Useful additional equipments |
|---|---|---|
| Incubator | Laminar flow hood | Low-temperature freezer |
| Microscope | Cell counter | Glassware washing machine |
| Sterilizer | Vacuum Pump | Colony counter |
| Washing up instrument | CO2 incubator | Closed-circuit machine |
How much does a tissue culture incubator cost?
Considering the purchase price (from about $3,000 to just south of $20,000), and an average usable life of about ten years, the projected growth rate is robust for a laboratory asset.
What does cell culture do?
Cell culture is a process where cells (animal or plant cells) are removed from the organism and introduced in to an artificial environment with favorable conditions for growth. This allows for researchers to study and learn more about the cells.
What is the primary equipment required for animal tissue culture laboratory?
Equipment: equipment like Laminar flow, sterilizer, CO2 cylinder, refrigerator, ware purifier, pipette washer, deep washing sink, liquid nitrogen freezer, inverted microscope, balance, a slow cooling device for freezing cells, centrifuge are the minimum requirement for animal cell tissue culture laboratory.
How do you start a cell culture?
Determine the correct dilution for obtaining the desired cell density, add the appropriate amount of fresh medium, and re-suspend the cells. Seed a predetermined amount of the cell suspension into a new culture vessel. Perform microscope observation. Transfer to a humidified CO2 incubator set to a temperature of 37 °C.
Do you need a large area for a tissue culture laboratory?
The tissue culture laboratory must have four basic environments: an office, a washing and media preparation room, a transference room, and a culture room, which require a minor to major grade of cleaning, as well as a minor to major restriction on personnel access.
How do you decontaminate a tissue culture hood?
Wipe down all surfaces in the hood with soapy water and then 70\% ethanol starting from the back and working your way forwards. Again, products that contain bleach are not ideal here as you do not want to damage the hood. Don’t forget the sash and the outside of the hood.
Why is there 5 CO2 in cell culture incubator?
5\%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.