Which of the following is an example of phagemid vector?

Phagemid vectors are plasmids having a small segment of a filamentous phage M-13, fd, or F1 phage capable to carry up to 10 kb passenger DNA. Examples: pEMBL series of plasmids pBluescript family plasmids.

What is the example of plasmid vector?

Some plasmids contain an M13 bacteriophage origin of replication and may be used to generate single-stranded DNA. These are called phagemid, and examples are the pBluescript series of cloning vectors.

What is a phagemid a hybrid vector phage vector plasmid vector viral vector?

Explanation: Phagemid is a hybrid of M13 phage and Pbr322 plasmid. Pembl8 is an example of phagemid which was created by transferring into a pUC8 a 1300 bp fragment of M13 genome.

What is the difference between cosmid and phagemid vector?

The key difference between Cosmid and Phagemid is on the type of sequences it contains. A Cosmid contains a cos site and a plasmid. Therefore, it is a hybrid vector while a Phagemid is a plasmid that contains an F1 origin of replication of the F1 phage.

What is F1 origin?

General description. A versatile cloning plasmid vector for the expression of genes in mammalian cells that can also be used to make single stranded DNA in bacterial cells that have been infected with bacteriophage F1. The F1 origin has been inserted into the PacI site and is now flanked by two PacI sites.

What is a plasmid vector?

Plasmid vectors are the vehicles used to drive recombinant DNA into a host cell and are a key component of molecular cloning; the procedure of constructing DNA molecules and introducing it into a host cell. Plasmid Vectors are a vital accessory to cloning.

What is plasmid or vector?

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell’s chromosomal DNA. Plasmids that are used experimentally for these purposes are called vectors. Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid.

What are cosmid vectors used for?

Cosmid vectors are designed to clone large fragments of DNA and to grow their DNA as a virus or as a plasmid. Cosmid vectors are used in homologous recombination between two different plasmids in the same cell and grown in both bacteria and animal cells.

What is a a phagemid vector?

A phagemid or phasmid is a DNA-based cloning vector, which has both bacteriophage and plasmid properties. These vectors carry, in addition to the origin of plasmid replication, an origin of replication derived from bacteriophage. , Writer..

What are the applications of a phagemid?

Phagemids are used in a variety of biotechnology applications; for example, they can be used in a molecular biology technique called ” Phage Display “. A phagemid is a plasmid that contains an f1 origin of replication from an f1 phage. It can be used as a type of cloning vector in combination with filamentous phage M13.

Why must a helper phage be coinfected with a phage?

As a result, cells containing a phagemid vector must be coinfected with a helper phage if ss DNA is desired. The helper phage converts the phagemids into ss DNA molecules that are then assembled into phage particles and secreted from the cell.

What is the origin of replication in phagemids?

Phagemids carry two replication origins, one a standard plasmid origin and the other derived from M13. The M13 origin is crucial for the synthesis of ss DNA. However, production of ss DNA requires enzymes and coat proteins coded by phage genes, which are lacking in phagemids.